attune flow cytometer - An Overview

attune flow cytometer - An Overview

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Info-enriched characterization of cell populations. Morphological data from pictures can incorporate towards the richness of flow cytometry information. By way of example, the figure displays an or else conventional apoptosis assay applying Annexin V and PI, incorporating mobile imaging to characterize cells in Just about every inhabitants to reveal morphologically distinctive features. These insights could not have been attained from multiplex staining by yourself.

No matter whether you’re a starter or an authority, investigate our methods to advance your exploration and know-how in flow cytometry.

Blend acoustic concentrating technological innovation with substantial-parameter Evaluation for versatile spectral unmixing and standard workflows.

Learn new insights into your experimental info by visualizing populations applying combined fluorescence and morphology information. Our software program interface wizards allow downsampling, concatenation (merged files) and dimensionality tools that happen to be designed in to the computer software for an intuitive, streamlined information Evaluation expertise.

*Pricing is indicative only and it is issue to vary. Remember to Make contact with your Agilent representative with any issues. The knowledge set forth herein is just not a suggestion or even a motivation, each of which happen to be topic to credit score approval, stipulations, and availability with Agilent. Agilent Fiscal Remedies is available in selected nations.

Listed here, an experienced user has gated singlets confidently. Following assessing the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate is made up of more than 4% aggregates.

Optimizing gating tactic. Even robust handbook singlet gating is mistake-susceptible and remains a subjective selection stage in Virtually all flow cytometry assays. Imaging can be utilized to substantiate and modify gates to include only solitary cells of fascination.

Our scientists will structure panels in your case as over and over you need—cost-free, no products purchase desired.

Early indications of this contamination experienced initially been dismissed as debris. Backgating to find out new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also allows you to use morphological functions to discover interesting subpopulations that might not be evident from flow cytometry info alone.

Maybe most significantly, these occasions comprise cells of Evidently unique phenotypes which may lead to incorrect conclusions with regards to double good functions (particularly in unusual populations).

AI-pushed automated impression Investigation While website using the Attune Cytometric Software program interprets function functions into distinctive morphology parameters that may be coupled with typical fluorescence and scatter parameters to reveal hugely reproducible inhabitants improvements and exclusive subsets.

We Formerly demonstrated the strength of imaging Vehicle-T/Ramos cell interactions. Permit’s examine just the populace of greatest desire, the double positive functions, to learn more. We are able to now use extended morphological parameters (in this article, circularity and skewness of intensity) to more study the options of such populations and refine gating on these functions, rising data robustness.

Leverage the strength of State-of-the-art engineering and innovation to speed up your discoveries. Rely on in the Attune Flow Cytometer relatives to deliver the reliability, pace, and precision that set your function aside.

No wash/no lyse leukocyte Assessment with imaging. Cells were being acquired from 24-hour-aged blood by dilution in one mM EDTA ( Expert UX/UI permits end users to teach AI designs for expanded applications. (A) The “Train Design” feature provides annotation instruments and genuine-time model training precision.

At the center of each and every Attune Flow Cytometer lies our innovative acoustic concentrating technological know-how, which utilizes sound waves to meticulously align cells within the flow mobile. This impressive technique surpasses regular hydrodynamic procedures, leading to a lot quicker processing speeds, exceptional details high-quality, and improved sample throughput.

The Attune Xenith cytometer is a significant-parameter spectral analyzer, supplying the two spectral unmixing and traditional flow capabilities for in depth and flexible cell Investigation.

Agilent Value Promise ensures you not less than a decade of instrument use through the date you buy. If not, We'll credit score you Together with the residual price of the system toward an upgraded design.

Here, we show that by gating methods utilizing picture-based quantitative parameters can distinguish interacting cells from coincident gatherings to a lot more precisely evaluate interacting cells.